Western Blotting (WB), Immunofluorescence (IF), ELISA
Specificity
At least two isoforms of FAM193B are known to exist, this antibody will detect both isoforms.
Purification
FAM193B Antibody is affinity chromatography purified via peptide column.
Immunogen
FAM193B antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human FAM193B. The immunogen is located within amino acids 120 - 170 of FAM193B.
FAM193B
Reactivity: Human
WB, IF, EIA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
FAM193B antibody can be used for detection of FAM193B by Western blot at 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
FAM193B Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
FAM193B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
FAM193B
(Family with Sequence Similarity 193, Member B (FAM193B))
IRIZIO antibody, Kiaa1931 antibody, family with sequence similarity 193 member B antibody, family with sequence similarity 193, member B antibody, FAM193B antibody, Fam193b antibody
Background
FAM193B Antibody: FAM193B, also known as IRIZIO, was initially identified as a protein that is upregulated in alveolar rhabdomyosarcoma (ARMS), a type of fast-growing tumor characterized by chromosomal translocations fusing the PAX3 or PAX7 gene with that of FOXO1. It has been suggested that, in addition to the PAX3-FOXO1 gene fusion, disruption of the Rb and p53 pathways is required for full ARMS tumorigenesis. In PAX3-FOXO1-expressing primary mouse fibroblasts that possessed a defective p53 pathway, FAM193B expression enabled the tumorigenic transformation, suggesting that FAM193B may contribute to rhabdomyosarcomagenesis in humans.