ZFX antibody (C-Term)

Catalog No. ABIN6991680

Product Details anti-ZFX Antibody

Target: ZFX (Zinc Finger Protein, X-Linked (ZFX))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZFX antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: ZFX antibody is human, mouse and rat reactive. Multiple isoforms of ZFX are known to exist. ZFX antibody will detect isoform 1 and 2 of ZFX.

Purification: ZFX Antibody is affinity chromatography purified via peptide column.

Immunogen: ZFX antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ZFX. The immunogen is located within the last 50 amino acids of ZFX.

Isotype: IgG

Application Details

Application Notes: ZFX antibody can be used for detection of ZFX by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 5 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ZFX Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ZFX antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ZFX

Target: ZFX (Zinc Finger Protein, X-Linked (ZFX))

Alternative Name: ZFX (ZFX Products)

Background: ZFX Antibody: The X-linked zinc finger protein ZFX is a member of the Kruppel C2H2-type zinc-finger protein family. The full-length protein contains an acidic transcriptional activation domain (AD), a nuclear localization sequence (NLS) and a DNA binding domain (DBD) consisting of 13 C2H2-type zinc fingers. Studies in mouse embryonic and adult hematopoietic stem cells showed that this gene was required as a transcriptional regulator for self-renewal of both stem cell types, but it was dispensable for growth and differentiation of their progeny.

Molecular Weight: Predicted: 89, 63 kDa

Observed: 63 kDa

Gene ID: 7543

NCBI Accession: NP_003401

UniProt: P17010