Nicalin antibody (C-Term)

Catalog No. ABIN6991672

Product Details anti-Nicalin Antibody

Target: Nicalin (NCLN)

Binding Specificity: AA 390-440, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nicalin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: NCLN Antibody is affinity chromatography purified via peptide column.

Immunogen: NCLN antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NCLN. The immunogen is located within amino acids 390 - 440 of NCLN.

Isotype: IgG

Application Details

Application Notes: NCLN antibody can be used for detection of NCLN by Western blot at 0.5 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 2.5 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: NCLN Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: NCLN antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Nicalin

Target: Nicalin (NCLN)

Alternative Name: NCLN (NCLN Products)

Background: NCLN Antibody: Nicalin, along with its binding partner Nomo, is a novel signaling antagonist to Nodals, signaling factors of the TGF-β, superfamily that play a key role in vertebrate development. Nicalin is distantly related to Nicastrin, a component of the Alzheimer's disease-associated gamma-secretase, and is thought to have a similar function. Another protein, TMEM147, has also been recently identified as associating with Nicalin and NOMO as part of the gamma-secretase-like complex.

Gene ID: 56926

NCBI Accession: NP_064555

UniProt: Q969V3