Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
Specificity
NKX2-4 antibody is predicted to not cross-react with other NK2 homeobox family members. At least two isoforms of NKX2-4 are known to exist, this antibody will detect both isoforms.
Purification
NKX2-4 Antibody is affinity chromatography purified via peptide column.
Immunogen
NKX2-4 antibody was raised against an 18 amino acid synthetic peptide near the center of human NKX2-4. The immunogen is located within amino acids 220 - 270 of NKX2-4.
NKX2-4
Reactivity: Human
WB, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
NKX2-4 antibody can be used for detection of NKX2-4 by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
NKX2-4 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
NKX2-4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
NKX2-4 Antibody: NKX2-4 (NK2 homeobox 4) is a member of a family of transcription factors that are involved in embryonic development and cell fate. NKX2-4 is closely related to NKX2-1 and NKX2-4 and is specifically localized to the ventral diencephalon. Recent experiments in Xenopus have suggested that the homeodomain protein XANF is necessary for the activation of NKX2-4, but does not directly regulate its expression.