Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Specificity
At least two isoforms of SLC35D3 are known to exist, this antibody will recognize both isoforms. SLC35D3 antibody is predicted to not cross-react with SLC35D1 or SLC35D2.
Purification
SLC35D3 Antibody is affinity chromatography purified via peptide column.
Immunogen
SLC35D3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SLC35D3. The immunogen is located within the last 50 amino acids of SLC35D3.
SLC35D3 antibody can be used for detection of SLC35D3 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 5 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
SLC35D3 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
SLC35D3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
SLC35D3
(Solute Carrier Family 35, Member D3 (SLC35D3))
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Background
SLC35D3 Antibody: The solute carrier family SLC35 consists of at least 17 proteins that act as nucleotide sugar transporters localized to the Golgi apparatus and endoplasmic reticulum. The novel protein SLC35D3 is highly homologous to SLC35D1 and SLC35D2, both of which transport UDP-glucuronic acid and UDP-N-acetylgalactosamine, suggesting that SLC35D3 is also involved in the transport of nucleotide sugars. It has been suggested that SLC35D3 regulates platelet dense granules, lysosome-related organelles which contain high concentrations of several biologically important low molecular weight molecules necessary for normal blood homeostasis.