Uroplakin 1B antibody (AA 110-160)

Catalog No. ABIN6991587

Product Details anti-Uroplakin 1B Antibody

Target: Uroplakin 1B (UPK1B)

Binding Specificity: AA 110-160

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Uroplakin 1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: At least two isoforms of UPK1B are known to exist, this antibody will recognize both isoforms. UPK1B antibody is predicted to not cross-react with other tetraspanin protein family members.

Purification: UPK1B Antibody is affinity chromatography purified via peptide column.

Immunogen: UPK1B antibody was raised against a 17 amino acid synthetic peptide near the center of human UPK1B. The immunogen is located within amino acids 110 - 160 of UPK1B.

Isotype: IgG

Application Details

Application Notes: UPK1B antibody can be used for detection of UPK1B by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: UPK1B Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: UPK1B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Uroplakin 1B

Target: Uroplakin 1B (UPK1B)

Alternative Name: UPK1B (UPK1B Products)

Background: UPK1B Antibody: Uroplakin-1B (UPK1B) is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. UPK1B is found in the asymmetrical unit membrane (AUM) where it can form a complex with other transmembrane 4 superfamily proteins and may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions.

Molecular Weight: Predicted: 29 kDa

Observed: 30 kDa

Gene ID: 7348

NCBI Accession: NP_008883

UniProt: O75841