CMYA5 antibody (C-Term)

Catalog No. ABIN6991554

Product Details anti-CMYA5 Antibody

Target: CMYA5 (Cardiomyopathy Associated 5 (CMYA5))

Binding Specificity: AA 3810-3860, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CMYA5 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: SPRYD2 antibody is predicted to not cross-react with other SPRYD protein family members. At least four isoforms of SPRYD2 are known to exist.

Purification: SPRYD2 Antibody is affinity chromatography purified via peptide column.

Immunogen: SPRYD2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPRYD2. The immunogen is located within amino acids 3810 - 3860 of SPRYD2.

Isotype: IgG

Application Details

Application Notes: SPRYD2 antibody can be used for detection of SPRYD2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SPRYD2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: SPRYD2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for CMYA5

Target: CMYA5 (Cardiomyopathy Associated 5 (CMYA5))

Alternative Name: SPRYD2 (CMYA5 Products)

Synonyms: cmya5l antibody, DKFZp469P2126 antibody, C5orf10 antibody, SPRYD2 antibody, TRIM76 antibody, 2310076E16Rik antibody, 2310076E21Rik antibody, AA420382 antibody, AI504003 antibody, Srfsd antibody, Tims antibody, sr553 antibody, cardiomyopathy associated 5 antibody, CMYA5 antibody, cmya5 antibody, Cmya5 antibody

Background: SPRYD2 Antibody: SPRYD2, also known as Myospryn, was originally identified as the muscle-specific partner of dysbindin and as a Mef-2 target gene. It is a large scaffolding protein localized to the Z-disc/costamere region of striated muscle. SPRYD2 includes a noncanonical tripartite motif (TRIM-like) that lacks the RING domain but consists of a B-box coiled coil (BBC), fibronectin 3 (FN3) repeats, and SPRY domains. SPRYD2 interacts with desmin and calcineurin, and it has been suggested to play a role in the biogenesis of lysosome and negatively regulates slow-fiber-type transformation and skeletal muscle regeneration. SPRYD2 is dysregulated in Duchenne muscular dystrophy.

Gene ID: 202333

NCBI Accession: NP_705838

UniProt: Q8N3K9