AP3S1 antibody

Catalog No. ABIN6991549

Product Details anti-AP3S1 Antibody

Target: AP3S1 (Adaptor-Related Protein Complex 3, sigma 1 Subunit (AP3S1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AP3S1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: At least three isoforms of AP3S1 are known to exist, this antibody will detect the two larger isoforms. AP3S1 antibody is predicted to not cross-react with other AP3 protein family members.

Purification: AP3S1 Antibody is affinity chromatography purified via peptide column.

Immunogen: AP3S1 antibody was raised against a 19 amino acid synthetic peptide near the center of human AP3S1. The immunogen is located within the first 50 amino acids of AP3S1.

Isotype: IgG

Application Details

Application Notes: AP3S1 antibody can be used for detection of AP3S1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: AP3S1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: AP3S1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for AP3S1

Target: AP3S1 (Adaptor-Related Protein Complex 3, sigma 1 Subunit (AP3S1))

Alternative Name: AP3S1 (AP3S1 Products)

Synonyms: CLAPS3 antibody, Sigma3A antibody, [s]3A antibody, adaptor related protein complex 3 sigma 1 subunit antibody, adaptor-related protein complex 3, sigma 1 subunit antibody, adaptor related protein complex 3 sigma 1 subunit S homeolog antibody, AP3S1 antibody, Ap3s1 antibody, ap3s1.S antibody

Background: AP3S1 Antibody: AP3S1 belongs to the adaptor complexes 3 (AP3) small subunit family which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. AP3 is an heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP3S1 Interacts with AGAP1 and may play an important role in carcinoma.

Gene ID: 1176

NCBI Accession: NP_001275

UniProt: Q92572