Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
MEIG1 Antibody is affinity chromatography purified via peptide column.
Immunogen
MEIG1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminal of human MEIG1. The immunogen is located within the last 50 amino acids of MEIG1.
MEIG1 antibody can be used for detection of MEIG1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
MEIG1 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
MEIG1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
MEIG1 Antibody: MEIG1, a murine gene first identified as a testis specific gene, is a chromosome/chromatin-binding protein initially expressed during meiosis but retained in the germ cell nucleus throughout later stages of spermatogenesis. MEIG1 is a highly conserved basal metazoan gene that is indispensable for mouse spermatogenesis. It is important for normal meiotic differentiation and absolutely crucial for terminal differentiation of spermatozoa. MEIG1 encodes two alternative transcripts, designated 2a2 and 11a2, both of which encode for a common ORF but differing in their 5' untranslated region (5'UTR) due to alternative promoters.