TYW3 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW3 are known to exist, this antibody will detect both isoforms.
Purification
TYW3 Antibody is affinity chromatography purified via peptide column.
Immunogen
TYW3 antibody was raised against a 17 amino acid synthetic peptide near the center of human TYW3. The immunogen is located within amino acids 160 - 210 of TYW3.
TYW3 antibody can be used for detection of TYW3 by Western blot at 1 μ,g/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
TYW3 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
TYW3 antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
TYW3
(tRNA-YW Synthesizing Protein 3 Homolog (TYW3))
C1orf171 antibody, 5230400J09Rik antibody, tRNA-yW synthesizing protein 3 homolog L homeolog antibody, tRNA-yW synthesizing protein 3 homolog antibody, tRNA-yW synthesizing protein 3 homolog (S. cerevisiae) antibody, tyw3.L antibody, TYW3 antibody, Tyw3 antibody
Background
TYW3 Antibody: TYW3 is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW3 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation.