PRR5L antibody (AA 220-270)

Catalog No. ABIN6991524

Product Details anti-PRR5L Antibody

Target: PRR5L (Proline Rich 5 Like (PRR5L))

Binding Specificity: AA 220-270

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRR5L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: At least three isoforms of PRR5L are known to exist, this antibody will recognize only isoforms a and b. PRR5L antibody will not cross-react with PPR5.

Purification: PRR5L Antibody is affinity chromatography purified via peptide column.

Immunogen: PRR5L antibody was raised against a 17 amino acid synthetic peptide near the center of human PRR5L. The immunogen is located within amino acids 220 - 270 of PRR5L.

Isotype: IgG

Application Details

Application Notes: PRR5L antibody can be used for detection of PRR5L by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PRR5L Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PRR5L antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PRR5L

Target: PRR5L (Proline Rich 5 Like (PRR5L))

Alternative Name: PRR5L (PRR5L Products)

Synonyms: PROTOR2 antibody, 2600010E01Rik antibody, 4833411O04Rik antibody, Protor-2 antibody, Protor2 antibody, RGD1309969 antibody, PRR5L antibody, FLJ14213 antibody, proline rich 5 like antibody, PRR5L antibody, Prr5l antibody, prr5l antibody

Background: PRR5L Antibody: Proline-rich protein 5-like (PRR5L), also known as Protor-2, is structurally similar to PRR5 and also binds to the mTOR complex 2 (mTORC2). PRR5L is not required for mTORC2 integrity or kinase activity, but dissociates from mTORC2 upon knockdown of tuberous sclerosis complex 1 (TSC1) and TSC2. Knockdown of PRR5L reduced apoptosis in mTORC2 deficient cells as well as cells where mTOR was active, suggesting that mTORC2-dissociated PRR5L may promote apoptosis, and thus play a role in the balance between cell growth and cell death.

Gene ID: 79899

NCBI Accession: NP_001153639

UniProt: Q6MZQ0