ELISA, Western Blotting (WB), Immunofluorescence (IF)
Specificity
At least three isoforms of ATP2C2 are known to exist. ATP2C2 antibody will not cross-react with ATP2C1
Purification
ATP2C2 Antibody is affinity chromatography purified via peptide column.
Immunogen
ATP2C2 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human ATP2C2. The immunogen is located within the first 50 amino acids of ATP2C2.
ATP2C2 antibody can be used for detection of ATP2C2 by Western blot at 1 μ,g/mL. Antibody can also be used for immunofluorescence starting at 20 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
ATP2C2 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
ATP2C2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
ATP2C2
(ATPase, Ca++ Transporting, Type 2C, Member 2 (ATP2C2))
KIAA0703 antibody, MGC146612 antibody, SPCA2 antibody, 1810010G06Rik antibody, Spca2 antibody, mKIAA0703 antibody, ATPase secretory pathway Ca2+ transporting 2 antibody, ATPase, Ca++ transporting, type 2C, member 2 S homeolog antibody, ATPase, Ca++ transporting, type 2C, member 2 antibody, ATP2C2 antibody, atp2c2.S antibody, atp2c2 antibody, Atp2c2 antibody
Background
ATP2C2 Antibody: ATP2C2, also known as secretory pathway Ca2+/Mn2+-ATPase (SPCA) 2, belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium from the cytosol to the Golgi lumen. Defects in the related gene ATP2C1 cause Hailey-Hailey disease, for which ATP2C2 does not compensate, suggesting that ATP2C2 plays other physiological roles. Unlike ATP2C1, ATP2C2 has a much more restricted expression pattern and displays a higher maximal turnover rate for overall Ca2+-ATPase reaction and a lower apparent affinity for cytosolic Ca2+ activation of phosphorylation. Overexpression of ATP2C2 in mammary tumors result a Ca2+ influx via the store-operated Ca2+ channel ORAI1 and independent of the STIM1 and STIM2 sensors.