ZNF219 antibody (N-Term)

Catalog No. ABIN6991505

Product Details anti-ZNF219 Antibody

Target: ZNF219 (Zinc Finger Protein 219 (ZNF219))

Binding Specificity: AA 90-140, N-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZNF219 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: At least two isoforms of ZFP219 are known to exist, this antibody will recognize both.

Purification: ZFP219 Antibody is affinity chromatography purified via peptide column.

Immunogen: ZFP219 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZFP219. The immunogen is located within amino acids 90 - 140 of ZFP219.

Isotype: IgG

Application Details

Application Notes: ZFP219 antibody can be used for detection of ZFP219 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ZFP219 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ZFP219 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ZNF219

Target: ZNF219 (Zinc Finger Protein 219 (ZNF219))

Alternative Name: ZFP219 (ZNF219 Products)

Synonyms: ZNF219 antibody, ZFP219 antibody, Znf219 antibody, 2010302A17Rik antibody, zinc finger protein 219 antibody, ZNF219 antibody, Zfp219 antibody

Background: ZFP219 Antibody: ZFP219 is a developmentally regulated member of the Kruppel-like zinc finger gene family that is thought to function as a transcriptional repressor. Yeast two-hybrid screening showed association with Sox9, a transcription factor that is essential for chondrogenesis. ZFP219 is specifically expressed in the developing limb buds and colocalizes with Sox9 in the nucleus. Knockdown of ZFP219 expression decreased Sox9-induced mRNA expression, and a dominant-negative mutant of ZFP219 inhibited Bmp2-induced chondrocyte differentiation, suggesting that ZFP219 plays an important role as a transcriptional partner of Sox9 in the regulation of chondrocyte differentiation.

Molecular Weight: Predicted: 80 kDa

Observed: 78 kDa

Gene ID: 51222

NCBI Accession: NP_001095142

UniProt: Q9P2Y4