Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Purification
FREM1 Antibody is affinity chromatography purified via peptide column.
Immunogen
FREM1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human FREM1. The immunogen is located within the last 50 amino acids of FREM1.
FREM1
Reactivity: Human
WB, ELISA, IF, IC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
FREM1 antibody can be used for detection of FREM1 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 20 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
FREM1 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
FREM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
FREM1
(FRAS1 Related Extracellular Matrix 1 (FREM1))
BNAR antibody, C9orf143 antibody, C9orf145 antibody, C9orf154 antibody, MOTA antibody, TILRR antibody, TRIGNO2 antibody, RGD1306981 antibody, BC037594 antibody, D430009N09 antibody, D630008K06 antibody, eye antibody, eyes2 antibody, heb antibody, qbrick antibody, FRAS1 related extracellular matrix 1 antibody, Fras1 related extracellular matrix 1 antibody, Fras1 related extracellular matrix protein 1 antibody, FREM1 antibody, Frem1 antibody
Background
FREM1 Antibody: FREM1 is a member of the FRAS1-related extracellular matrix protein family and is thought to play a role in craniofacial and renal development. FREM1 functions as an extracellular matrix protein that is essential for epidermal adhesion during embryogenesis and may also participate in epidermal differentiation. It is recognized by cells in the embryonic skin and hair follicles through different members of the integrin family. Deficiency in the Fras1/Frem genes gives rise to the bleb phenotype, which is equivalent to the human hereditary disorder Fraser syndrome.