ANAPC13 antibody

Catalog No. ABIN6991331

Product Details anti-ANAPC13 Antibody

Target: ANAPC13 (Anaphase Promoting Complex Subunit 13 (ANAPC13))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANAPC13 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purification: APC13 Antibody is affinity chromatography purified via peptide column.

Immunogen: APC13 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC13. The immunogen is located within the last 50 amino acids of APC13.

Isotype: IgG

Application Details

Application Notes: APC13 antibody can be used for detection of APC13 by Western blot at 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: APC13 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: APC13 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ANAPC13

Target: ANAPC13 (Anaphase Promoting Complex Subunit 13 (ANAPC13))

Alternative Name: APC13 (ANAPC13 Products)

Synonyms: APC13 antibody, SWM1 antibody, 1810004D07Rik antibody, anaphase promoting complex subunit 13 antibody, ANAPC13 antibody, Bm1_31995 antibody, LOAG_01662 antibody, Anapc13 antibody

Background: APC13 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC13 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC13 promotes the stable association of APC3/Cdc27 and APC6/Cdc16 with the APC/C.

Molecular Weight: Predicted: 15 kDa

Observed: 14 kDa

Gene ID: 25847

NCBI Accession: NP_056206

UniProt: Q9BS18