FAM33A antibody (N-Term)

Catalog No. ABIN6991191

Product Details anti-FAM33A Antibody

Target: FAM33A (Spindle and Kinetochore-Associated Protein 2 (FAM33A))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAM33A antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purification: SKA2 Antibody is affinity chromatography purified via peptide column.

Immunogen: SKA2 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human SKA2. The immunogen is located within the first 50 amino acids of SKA2.

Isotype: IgG

Application Details

Application Notes: SKA2 antibody can be used for detection of SKA2 by Western blot at 0.5 - 1 μ,g/mL.

Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SKA2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: SKA2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for FAM33A

Target: FAM33A (Spindle and Kinetochore-Associated Protein 2 (FAM33A))

Alternative Name: SKA2 (FAM33A Products)

Synonyms: FAM33A antibody, 1110001A07Rik antibody, C78640 antibody, Fam33a antibody, RGD1307084 antibody, fam33a antibody, spindle and kinetochore associated complex subunit 2 antibody, Spindle and kinetochore-associated protein 2 antibody, SKA2 antibody, Ska2 antibody, ska2 antibody

Background: SKA2 Antibody: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing. SKA2 has also been shown to interact with glucocorticoid receptors and to be involved in glucocorticoid signaling and cell proliferation.

Gene ID: 348235

NCBI Accession: NP_872426

UniProt: Q8WVK7

Pathways: M Phase