Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
Specificity
Multiple isoforms of OTUD4 are known to exist. OTUD4 antibody is predicted to not cross-react with other members of the OTUD family.
Purification
OTUD4 Antibody is affinity chromatography purified via peptide column.
Immunogen
OTUD4 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human OTUD4. The immunogen is located within the last 50 amino acids of OTUD4.
OTUD4
Reactivity: Human, Mouse
WB, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
OTUD4 antibody can be used for detection of OTUD4 by Western blot at 0.25 - 0.5 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
OTUD4 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
OTUD4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
OTUD4 Antibody: OTUD4, also known as HIV-1 induced protein HIN-1, is a member of the OTU (ovarian tumor) domain containing cysteine protease superfamily, in which the OUT domain generally confers deubiquitinase activity. At least three isoforms of OTUD4 are known to exist, and the smallest of these isoforms are only expressed in HIV-1-infected cells (provided by RefSeq).