PIGY antibody (AA 50-100)

Catalog No. ABIN6991033

Product Details anti-PIGY Antibody

Target: PIGY (Phosphatidylinositol Glycan Anchor Biosynthesis, Class Y (PIGY))

Binding Specificity: AA 50-100

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGY antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: This antibody only detects isoform 1 of PIG-Y.

Purification: PIG-Y Antibody is affinity chromatography purified via peptide column.

Immunogen: PIG-Y antibody was raised against a 16 amino acid synthetic peptide near the center of human PIG-Y. The immunogen is located within amino acids 50 - 100 of PIG-Y.

Isotype: IgG

Application Details

Application Notes: PIG-Y antibody can be used for detection of PIG-Y by Western blot at 1 - 2 μ,g/mL. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PIG-Y Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PIG-Y antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PIGY

Target: PIGY (Phosphatidylinositol Glycan Anchor Biosynthesis, Class Y (PIGY))

Alternative Name: PIG-Y (PIGY Products)

Synonyms: PIG-Y antibody, pig-y antibody, Prey antibody, Pyurf antibody, prey antibody, 1810008A14Rik antibody, Pigy antibody, phosphatidylinositol glycan anchor biosynthesis class Y antibody, phosphatidylinositol glycan anchor biosynthesis, class Y antibody, Pigy upstream reading frame S homeolog antibody, phosphatidylinositol glycan anchor biosynthesis, class Y-like antibody, PIGY antibody, pigy antibody, Pigy antibody, pyurf.S antibody, Pigyl antibody

Background: PIG-Y Antibody: Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA.

Molecular Weight: Predicted: 8 kDa

Observed: 16 kDa

Gene ID: 84992

NCBI Accession: NP_116295

UniProt: Q3MUY2

Pathways: Inositol Metabolic Process