TOM70 antibody (N-Term)

Catalog No. ABIN6991030

Product Details anti-TOM70 Antibody

Target: TOM70 (TOMM70A) (Translocase of Outer Mitochondrial Membrane 70 (TOMM70A))

Binding Specificity: AA 50-100, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOM70 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: At least two isoforms of TOM70 are known to exist, this antibody will only detect the larger isoform.

Purification: TOM70 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-TOM70 antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human TOM70. The immunogen is located within amino acids 50-100 of TOM70.

Isotype: IgG

Application Details

Application Notes: WB: 1-4 μ,g/mL, IHC: 2.5 μ,g/mL, IF: 20 μ,g/mL.

Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TOM70 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TOM70 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for TOM70

Target: TOM70 (TOMM70A) (Translocase of Outer Mitochondrial Membrane 70 (TOMM70A))

Alternative Name: TOM70 (TOMM70A Products)

Background: TOM70 Antibody: The translocase of outer mitochondrial membrane (TOM) complex is a multisubunit complex involved in the recognition, unfolding, and translocation of preproteins into the mitochondria. TOM70, an important member of the TOM complex, contains a tetratricopeptide repeat domain similar to those found in cytosolic chaperones such as Hsp90 and Hsc70 and provides a docking site for these proteins. This interaction is thought to be a critical first step in the TOM70-dependent mitochondrial import, followed by contact between the preprotein and TOM70. After targeting to TOM70, preproteins are translocated through the outer membrane via the TOM40 import pore complex. The precise mechanism of how preproteins progress from TOM70 to TOM40 to full translocation is still unclear.

Molecular Weight: Predicted: 68kD

Observed: 72 kD kDa

Gene ID: 9868

NCBI Accession: NP_055635

UniProt: O94826

Pathways: SARS-CoV-2 Protein Interactome