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SPRED3 antibody (AA 140-190)

SPRED3 Reactivity: Human WB, ELISA, IHC (p), IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6990998
  • Target See all SPRED3 Antibodies
    SPRED3 (Sprouty-Related, EVH1 Domain Containing 3 (SPRED3))
    Binding Specificity
    • 8
    • 8
    • 7
    • 1
    AA 140-190
    Reactivity
    • 16
    • 9
    Human
    Host
    • 16
    Rabbit
    Clonality
    • 16
    Polyclonal
    Conjugate
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    This SPRED3 antibody is un-conjugated
    Application
    • 16
    • 13
    • 12
    • 4
    • 2
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
    Specificity
    This Spred3 antibody is predicted to have no cross-reactivity to Spred1 or Spred2.
    Purification
    Spred3 Antibody is affinity chromatography purified via peptide column.
    Immunogen
    Spred3 antibody was raised against a 16 amino acid synthetic peptide near the center of the human Spred3. The immunogen is located within amino acids 140 - 190 of Spred3.
    Isotype
    IgG
    Top Product
    Discover our top product SPRED3 Primary Antibody
  • Application Notes
    Spred3 antibody can be used for detection of Spred3 by Western blot at 2 - 4 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

    Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Spred3 Antibody is supplied in PBS containing 0.02 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C,4 °C
    Storage Comment
    Spred3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
  • Target
    SPRED3 (Sprouty-Related, EVH1 Domain Containing 3 (SPRED3))
    Alternative Name
    Spred3 (SPRED3 Products)
    Synonyms
    si:ch211-255l12.2 antibody, RGD1311713 antibody, Eve-3 antibody, spred-3 antibody, AW047143 antibody, D130060H24Rik antibody, Spred-3 antibody, sprouty related EVH1 domain containing 3 antibody, sprouty-related, EVH1 domain containing 3 antibody, SPRED3 antibody, spred3 antibody, Spred3 antibody
    Background
    Spred3 Antibody: Spred3 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1 and Spred2, Spred3 contains and EVH1 domain in its amino terminus and a Sprouty-related cysteine-rich (SPR) domain in its C-terminus, but does not possess a functional c-kit binding domain (KBD). The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Although Spred3 was initially reported to be expressed in brain, at least one report also indicates that Spred3 can be found in liver. At least two isoforms of Spred3 are known to exist.
    Molecular Weight
    Predicted: 45 kDa

    Observed: 45 kDa
    Gene ID
    399473
    NCBI Accession
    NP_001035987
    UniProt
    Q2MJR0
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