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SPRED1 antibody (AA 250-300)

SPRED1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6990996
  • Target See all SPRED1 Antibodies
    SPRED1 (Sprouty-Related, EVH1 Domain Containing 1 (SPRED1))
    Binding Specificity
    • 7
    • 7
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 250-300
    Reactivity
    Human, Mouse, Rat
    Host
    • 37
    • 7
    Rabbit
    Clonality
    • 37
    • 7
    Polyclonal
    Conjugate
    • 20
    • 6
    • 5
    • 5
    • 4
    • 4
    This SPRED1 antibody is un-conjugated
    Application
    • 39
    • 33
    • 20
    • 7
    • 6
    • 3
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
    Specificity
    This Spred1 antibody is predicted to have no cross-reactivity to Spred2 or Spred3.
    Purification
    Spred1 Antibody is affinity chromatography purified via peptide column.
    Immunogen
    Spred1 antibody was raised against a 14 amino acid synthetic peptide near the center of the human Spred1. The immunogen is located within amino acids 250 - 300 of Spred1.
    Isotype
    IgG
    Top Product
    Discover our top product SPRED1 Primary Antibody
  • Application Notes
    Spred1 antibody can be used for detection of Spred1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

    Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Spred1 Antibody is supplied in PBS containing 0.02 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C,4 °C
    Storage Comment
    Spred1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
  • Target
    SPRED1 (Sprouty-Related, EVH1 Domain Containing 1 (SPRED1))
    Alternative Name
    Spred1 (SPRED1 Products)
    Synonyms
    fb14c08 antibody, fj51c03 antibody, zgc:85707 antibody, wu:fb14c08 antibody, wu:fj51c03 antibody, Xspred1 antibody, Xtspred1 antibody, 5730461F13Rik antibody, AL024345 antibody, AW047647 antibody, NFLS antibody, hSpred1 antibody, spred-1 antibody, sprouty-related, EVH1 domain containing 1 antibody, sprouty related EVH1 domain containing 1 antibody, sprouty related, EVH1 domain containing 1 antibody, sprouty related, EVH1 domain containing 1 L homeolog antibody, SPRED1 antibody, sprouty protein with EVH-1 domain 1, related sequence antibody, spred1 antibody, SPRED1 antibody, spred1.L antibody, Bm1_51610 antibody, Spred1 antibody
    Background
    Spred1 Antibody: The Ras-MAP kinase pathway is essential for the differentiation of neuronal cells and myocytes, it is inhibited by Spred1, a member of the Sprouty family of proteins. Spred1 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS).
    Molecular Weight
    Predicted: 49 kDa

    Observed: 498 kDa
    Gene ID
    161742
    NCBI Accession
    NP_689807
    UniProt
    Q7Z699
    Pathways
    Positive Regulation of Response to DNA Damage Stimulus
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