Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
Purification
OVGP1 Antibody is affinity chromatography purified via peptide column.
Immunogen
OVGP1 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human OVGP1. The immunogen is located within amino acids 60 - 110 of OVGP1.
OVGP1
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
Biotin
Application Notes
OVGP1 antibody can be used for the detection of OVGP1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 2.5 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
OVGP1 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
OVGP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
OVGP1 Antibody: Oviductins belong to a family of glycoproteins that have been suggested to play several roles during the early processes of reproduction. OVGP1 is a large carbohydrate-rich, epithelial glycoprotein with numerous O-glycosylation sites within threonine, serine, and proline-rich tandem repeats. The protein is secreted from non-ciliated oviductal epithelial cells and associates with ovulated oocytes, blastomeres, and spermatozoan acrosomal regions. At least two isoforms of OVGP are known to exist.
Molecular Weight
Predicted: 75 kDa
Observed: Due to extensive post-translational modification, OVGP1 migrates at ~140 kDa in SDS-PAGE.