NANOS2 antibody (N-Term)

Catalog No. ABIN6990924

Product Details anti-NANOS2 Antibody

Target: NANOS2 (Nanos Homolog 2 (NANOS2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NANOS2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purification: Nanos2 Antibody is affinity chromatography purified via peptide column.

Immunogen: Nanos2 antibody was raised against a 13 amino acid synthetic peptide near the amino terminus of the human Nanos2. The immunogen is located within the first 50 amino acids of Nanos2.

Isotype: IgG

Application Details

Application Notes: Nanos2 antibody can be used for detection of Nanos2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Nanos2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Nanos2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for NANOS2

Target: NANOS2 (Nanos Homolog 2 (NANOS2))

Alternative Name: Nanos2 (NANOS2 Products)

Synonyms: NOS2 antibody, RGD1562436 antibody, nos2 antibody, nanos C2HC-type zinc finger 2 antibody, nanos homolog 2 (Drosophila) antibody, nanos 2 antibody, nanos2 protein antibody, NANOS2 antibody, Nanos2 antibody, nanos2 antibody

Background: Nanos2 Antibody: Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In Drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3-null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3-null background. Nanos3 expression can not rescue Nanos2-null defects. This Nanos2 antibody will not cross-react with either Nanos 1 or Nanos3.

Gene ID: 339345

UniProt: P60321

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Stem Cell Maintenance