DLGAP1 antibody (AA 640-690)

Catalog No. ABIN6990914

Product Details anti-DLGAP1 Antibody

Target: DLGAP1 (Discs, Large (Drosophila) Homolog-Associated Protein 1 (DLGAP1))

Binding Specificity: AA 640-690

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLGAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: At least three isoforms of SAPAP1 are known to exist. This SAPAP1 antibody will not cross-react with other SAPAP proteins.

Purification: SAPAP1 Antibody is affinity chromatography purified via peptide column.

Immunogen: SAPAP1 antibody was raised against a 18 amino acid synthetic peptide from near the center of human SAPAP1. The immunogen is located within amino acids 640 - 690 of SAPAP1.

Isotype: IgG

Application Details

Application Notes: SAPAP1 antibody can be used for detection of SAPAP1 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SAPAP1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: SAPAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for DLGAP1

Target: DLGAP1 (Discs, Large (Drosophila) Homolog-Associated Protein 1 (DLGAP1))

Alternative Name: SAPAP1 (DLGAP1 Products)

Synonyms: DKFZp469D0713 antibody, gkap antibody, sapap1 antibody, DAP-1 antibody, DAP-1-ALPHA antibody, DAP-1-BETA antibody, GKAP antibody, SAPAP1 antibody, hGKAP antibody, 4933422O14Rik antibody, 9630002F18 antibody, AI845682 antibody, AI848168 antibody, BB075781 antibody, D17Bwg0511e antibody, GKAP/SAPAP antibody, GKPA/SAPAP antibody, Gkap antibody, Sapap1 antibody, mKIAA4162 antibody, DLG associated protein 1 antibody, discs, large (Drosophila) homolog-associated protein 1 antibody, disks large-associated protein 1 antibody, discs, large homolog-associated protein 1 antibody, discs, large (Drosophila) homolog-associated protein 1b antibody, DLGAP1 antibody, LOC100408093 antibody, LOC100548434 antibody, dlgap1 antibody, Dlgap1 antibody, dlgap1b antibody

Background: SAPAP1 Antibody: SAP90/PSD-95-associated protein 1 (SAPAP1, also known as DLGAP1 or GKAP) is a member of a protein family whose members specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. Like the other SAPAP proteins, SAPAP1 is thought to be an adaptor protein that also interacts with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). SAPAP1 mRNA is targeted to cell bodies in a similar manner to SAPAP2 and -4, whereas SAPAP3 mRNA is detected mainly in cell bodies. SAPAP1 protein however, is targeted to the synapse and is not reliant on the synaptic localization of PSD-95 or the synaptic scaffolding molecule (S-SCAM).

Gene ID: 9229

NCBI Accession: NP_004737

UniProt: O60500