ARHGEF17 antibody (AA 910-960)

Catalog No. ABIN6990812

Product Details anti-ARHGEF17 Antibody

Target: ARHGEF17 (rho Guanine Nucleotide Exchange Factor (GEF) 17 (ARHGEF17))

Binding Specificity: AA 910-960

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF17 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: TEM4 Antibody is affinity chromatography purified via peptide column.

Immunogen: TEM4 antibody was raised against a 18 amino acid synthetic peptide near the center of human TEM4. The immunogen is located within amino acids 910 - 960 of TEM4.

Isotype: IgG

Application Details

Application Notes: TEM4 antibody can be used for detection of TEM4 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TEM4 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TEM4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ARHGEF17

Target: ARHGEF17 (rho Guanine Nucleotide Exchange Factor (GEF) 17 (ARHGEF17))

Alternative Name: TEM4 (ARHGEF17 Products)

Background: TEM4 Antibody: Rho GTPases, which are activated by specific guanine-nucleotide exchange factors (GEFs), play pivotal roles in several cellular functions. TEM4, encoding a protein containing 1510 amino acids, contains a RhoGEF-specific Dbl homology (DH) domain but lacks their typical pleckstrin homology domain. TEM4 is a Rho-specific GEF with novel structural and regulatory properties and predominant expression in the heart. It couples tyrosine kinase signals with the activation of the rho/rac GTPases, thus leading to cell differentiation and/or proliferation. Elevated levels of TEM4, TEM5, TEM6, TEM7 and TEM7R were also raised in breast cancer tissues. TEM4 could also prove to be useful targets therapeutically.

Molecular Weight: Predicted: 220 kDa

Observed: 200 kDa

Gene ID: 9828

UniProt: Q96PE2