MANF antibody (C-Term)

Catalog No. ABIN6990751

Product Details anti-MANF Antibody

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MANF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody does not cross-react with CDNF. The specificity of this antibody was further verified by MANF specific knockout. MANF signal was not detected in MANF knockout HEK293T cells as compared to that in control wild type cells.

Purification: MANF Antibody is affinity chromatography purified via peptide column.

Grade: KO Validated

Immunogen: MANF antibody was raised against a 12 amino acid synthetic peptide from near the carboxy terminus of human MANF. The immunogen is located within the last 50 amino acids of MANF.

Isotype: IgG

Application Details

Application Notes: WB: 0.125-2 μ,g/mL, IHC: 2.5 μ,g/mL, IF: 20 μ,g/mL

Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: MANF Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: MANF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for MANF

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Alternative Name: MANF (MANF Products)

Background: MANF Antibody: MANF, also known as ARMET, was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. More recently it was identified as a mesencephalic astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons, similar to glial cell line-derived neurotrophic factor (GDNF) and CDNF. In rat brain slices, MANF enhanced nigral gamma-aminobutyric acid release. Like GDNF and CDNF, MANF has selective neuroprotective activity for dopaminergic neurons suggesting that it may be indicated for the treatment of Parkinson's disease. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress.

Molecular Weight: Predicted: 20kD

Observed: 18 kD kDa

Gene ID: 7873

UniProt: P55145