Stim2 antibody (AA 140-190)

Catalog No. ABIN6990746

Product Details anti-Stim2 Antibody

Target: Stim2 (Stromal Interaction Molecule 2 (Stim2))

Binding Specificity: AA 140-190

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Stim2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purification: STIM2 Antibody is affinity chromatography purified via peptide column.

Immunogen: STIM2 antibody was raised against a 17 amino acid synthetic peptide from near the center of human STIM2. The immunogen is located within amino acids 140 - 190 of STIM2.

Isotype: IgG

Application Details

Application Notes: STIM2 antibody can be used for detection of STIM2 by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: STIM2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: STIM2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Stim2

Target: Stim2 (Stromal Interaction Molecule 2 (Stim2))

Alternative Name: STIM2 (Stim2 Products)

Synonyms: STIM2 antibody, stromal interaction molecule 2 antibody, STIM2 antibody, stim2 antibody, Stim2 antibody

Background: STIM2 Antibody: In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++-release-activated Ca++ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry. At least three isoforms of STIM2 are known to exist. This STIM2 antibody is predicted to have no cross-reactivity to STIM1.

Gene ID: 57620

NCBI Accession: NP_065911

UniProt: Q9P246

Pathways: TCR Signaling, BCR Signaling