PHAP I antibody (C-Term)
Reactivity: Human, Mouse, Rat
WB, ELISA, ICC, IF
Host: Rabbit
Polyclonal
unconjugated
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- Target See all PHAP I products
- PHAP I
- Binding Specificity
- C-Term
- Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PHAP I antibody is un-conjugated
- Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
- Specificity
- This polyclonal antibody has no cross-reaction to PHAP I2a and PHAP III.
- Purification
- PHAP I Antibody is affinity chromatography purified via peptide column.
- Immunogen
- Anti-PHAP I antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human PHAP I. The immunogen is located within the last 50 amino acids of PHAP I.
- Isotype
- IgG
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- Application Notes
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WB: 2-4 μ,g/mL, ICC: 2 μ,g/mL, IF: 10 ?g/mL.
Antibody validated: Western Blot in human, mouse and rat samples, Immunocytochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PHAP I Antibody is supplied in PBS containing 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,4 °C
- Storage Comment
- PHAP I antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- Target
- PHAP I
- Abstract
- PHAP I Products
- Background
- PHAP I Antibody: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
- Molecular Weight
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Predicted: 29kD
Observed: 29 kD kDa - Gene ID
- 8125
- UniProt
- P39687
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