SKI antibody (N-Term)

Catalog No. ABIN6990332

Product Details anti-SKI Antibody

Target: SKI (SKI Proto-Oncogene (SKI))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKI antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purification: Ski Antibody is affinity chromatography purified via peptide column.

Immunogen: Ski antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Ski. The immunogen is located within the first 50 amino acids of Ski.

Isotype: IgG

Application Details

Application Notes: SkiP antibody can be used for detection of Ski by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 20 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Ski Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Ski antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for SKI

Target: SKI (SKI Proto-Oncogene (SKI))

Alternative Name: Ski (SKI Products)

Background: Ski Antibody: TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Ski functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. Ski will also bind to the Smad proteins specific to bone morphogenic proteins (BMPs) and block BMP signaling in mammalian cells.

Gene ID: 6497

UniProt: P12755

Pathways: Stem Cell Maintenance, Tube Formation, Skeletal Muscle Fiber Development