MADD antibody (C-Term)

Catalog No. ABIN6990256

Product Details anti-MADD Antibody

Target: MADD (MAP-Kinase Activating Death Domain (MADD))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MADD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Purification: MADD Antibody is Antibody is DEAE purified.

Immunogen: MADD antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human MADD. The immunogen is located within the last 50 amino acids of MADD.

Isotype: IgG

Application Details

Application Notes: MADD antibody can be used for detection of MADD by Western blot at 1 - 2 mg/mL. 200 to 220 kDa bands should be detected. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: MADD Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: MADD antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for MADD

Target: MADD (MAP-Kinase Activating Death Domain (MADD))

Alternative Name: MADD (MADD Products)

Synonyms: MADD antibody, MGC145371 antibody, DENN antibody, IG20 antibody, RAB3GEP antibody, 9630059K23Rik antibody, MAP kinase activating death domain antibody, MAP-kinase activating death domain antibody, MADD antibody, madd antibody, Madd antibody

Background: MADD Antibody: MAP kinase-activating death domain protein (MADD) was initially identified as the type 1 tumor necrosis factor receptor (TNFR1) associated protein though their death domains. Overexpression of MADD activates MAP kinases ERK and JNK and induces the phosphorylation of cytosolic phospholipase A2. MADD shares 98 % identity with DENN (for differentially expressed in neoplastic vs. normal cells), which was recently identified as a substrate for c-jun N-terminal kinase 3 (JNK3). MADD has greater than 94 % overall identity to a GDP/GTP exchange protein Rab3-GEP. MADD is 87 % identical to KIAA0358, a brain protein of unknown function. Identification of MADD as a component of the TNFR1 signaling complex and the similarity between MADD and Rab3-GEP provides a connection between TNFR1 activation and downstream MAP kinase activity through a guanine-nucleotide exchange protein.

Molecular Weight: 200 to 220 kDa

Gene ID: 8567

UniProt: Q8WXG6

Pathways: Caspase Cascade in Apoptosis