SARS-CoV-2 NSP5 (3CL-Pro) antibody (N-Term)

Catalog No. ABIN6990134

Product Details anti-SARS-CoV-2 NSP5 (3CL-Pro) Antibody

Target: SARS-CoV-2 NSP5 (3CL-Pro) (3CL-PRO, M-Pro) (SARS-CoV-2 3C-Like Proteinase (NSP5) (3CL-PRO, M-Pro))

Binding Specificity: N-Term

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SARS-CoV-2 NSP5 (3CL-Pro) antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Purification: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of SARS-CoV-2 (COVID-19) 3CL-Pro (NSP5) protein. The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) 3CL-Pro (NSP5) protein.

Isotype: IgG

Application Details

Application Notes: IHC: 0.5 μ,g/mL, WB: 0.5 μ,g/mL

Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody can detect 2 ng of free peptide at 1 μ,g/mL in ELISA. It can detect SARS-CoV-2 NSP5 recombinant protein by ELISA and WB. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody can be stored at 4 ° C for three months and -20 ° C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for SARS-CoV-2 NSP5 (3CL-Pro)

Target: SARS-CoV-2 NSP5 (3CL-Pro) (3CL-PRO, M-Pro) (SARS-CoV-2 3C-Like Proteinase (NSP5) (3CL-PRO, M-Pro))

Alternative Name: SARS-CoV-2 3CL-PRO (3CL-PRO, M-Pro Products)

Synonyms: pp1ab antibody, ORF1a polyprotein;ORF1ab polyprotein antibody, ORF1ab antibody

Target Type: Viral Protein

Background: Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019-20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. 3C- like proteinase (NSP5) cleaves the C-terminus of replicase polyprotein at 11 sites (3,4,5). It recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. It also able to bind an ADP-ribose-1''-phosphate (ADRP) (5).

Gene ID: 43740578

NCBI Accession: YP_009742612

UniProt: P0DTC1