This SARS-CoV-2 NSP5 (3CL-Pro) antibody is un-conjugated
Application
ELISA, Western Blotting (WB)
Purification
SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody is affinity chromatography purified via peptide column.
Immunogen
Anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody was raised against a peptide corresponding to 15 amino acids in the center of SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) protein. The immunogen is located within 80-130 amino acids of the SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) protein.
Antibody validated: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody can detect 2 ng of free peptide at 1 μ,g/mL in ELISA.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody can be stored at 4 ° C for three months and -20 ° C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019-20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. 3C- like proteinase (NSP5) cleaves the C-terminus of replicase polyprotein at 11 sites (3,4,5). It recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. It also able to bind an ADP-ribose-1''-phosphate (ADRP) (5).