EIF3B antibody

Catalog No. ABIN6989663

Product Details anti-EIF3B Antibody

Target: EIF3B (Eukaryotic Translation Initiation Factor 3, Subunit B (EIF3B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EIF3B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Synthetic Peptide within N terminal human eIF3B.

Clone: 5H10

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for EIF3B

Target: EIF3B (Eukaryotic Translation Initiation Factor 3, Subunit B (EIF3B))

Alternative Name: eIF3B (EIF3B Products)

Background:

Synonyms: eIF-3-eta antibody, eIF3 eta antibody, eIF3 p110 antibody, eIF3 p116 antibody, EIF3-ETA antibody, EIF3-P110 antibody, EIF3-P116 antibody, eIF3b antibody, EIF3B_HUMAN antibody, EIF3S9 antibody, EIF3S9, formerly antibody, Eukaryotic translation initiation factor 3, subunit B antibody, Eukaryotic translation initiation factor 3 subunit 9 antibody, eukaryotic translation initiation factor 3 subunit 9 eta antibody, Eukaryotic translation initiation factor 3 subunit B antibody, eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD) antibody, eukaryotic translation initiation factor 3, subunit 9 eta, 116 kDa antibody, eukaryotic translation initiation factor 3, subunit 9, formerly antibody, hPrt1 antibody, Protein synthesis defective at 36 degrees celsius 1, S. cerevisiae, homolog of antibody, PRT1 antibody, Prt1 homolog antibody

Background: eIF3b expression relates to human bladder and prostate cancer prognosis, is required for tumor growth, and thus a candidate therapeutic target.The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. Association of eIF3 proteins with the 40S ribosomal subunit stabilizes eIF2-GTP-Met-tRNAiMet complex association and mRNA binding, and promotes dissociation of 80S ribosomes into 40S and 60S subunits, thereby promoting the assembly of the pre-initiation complex. Overexpression of eIF3 proteins is common in several cancers, suggesting a role for eIF3 proteins in tumorigenesis.

Gene ID: 8662

UniProt: P55884

Pathways: Ribonucleoprotein Complex Subunit Organization