YWHAB/YWHAZ antibody (pSer184, pSer186)

Catalog No. ABIN6989478

Product Details anti-YWHAB/YWHAZ Antibody

Target: YWHAB/YWHAZ (14-3-3 beta/zeta (YWHAB/YWHAZ))

Binding Specificity: pSer184, pSer186

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YWHAB/YWHAZ antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthesised phosphopeptide derived from human 14-3-3 beta + zeta around the phosphorylation site of Ser186 / Ser184

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for YWHAB/YWHAZ

Target: YWHAB/YWHAZ (14-3-3 beta/zeta (YWHAB/YWHAZ))

Alternative Name: 14-3-3 beta + zeta (YWHAB/YWHAZ Products)

Synonyms: 14-3-3z antibody, 14-3-3zeta antibody, kcip-1 antibody, ywhaq antibody, Ywhaz antibody, ACYPI003154 antibody, 1433z antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta antibody, 14-3-3 protein zeta antibody, ywhaz antibody, 14-3-3zeta antibody, YWHAZ antibody, 1433z antibody

Background:

Synonyms: 14-3-3 beta + zeta (phospho S184 + S186), 14-3-3 beta + zeta (phospho S186), 14-3-3 beta + zeta (phospho Ser186), p-14-3-3 beta + zeta (Ser186) 14 3 3 protein beta, 14 3 3 protein beta/alpha, 14 3 3 protein zeta/delta, 14 3 3 zeta, HS1, KCIP1, YWHAB, YWHAZ.

Background: Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation amoung many other functions.

Gene ID: 7529

UniProt: P31946