TRBP antibody (AA 21-120) (Cy3)

Catalog No. ABIN6983417

Product Details anti-TRBP Antibody (Cy3)

Target: TRBP (TARBP2P) (TAR (HIV-1) RNA Binding Protein 2 Pseudogene (TARBP2P))

Binding Specificity: AA 21-120

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRBP antibody is conjugated to Cy3

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TRBP

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TRBP

Target: TRBP (TARBP2P) (TAR (HIV-1) RNA Binding Protein 2 Pseudogene (TARBP2P))

Alternative Name: TRBP (TARBP2P Products)

Synonyms: TRBP antibody, TARBP2P1, RISC loading complex RNA binding subunit pseudogene 1 antibody, TARBP2P1 antibody

Background:

Synonyms: LOQS, Prbp, RISC loading complex subunit TARBP2, RISC-loading complex subunit tarbp2, TAR (HIV 1) RNA binding protein 2, TAR (HIV) RNA binding protein 2, TAR (HIV) RNA-binding protein TRBP1, TAR RNA Binding Protein 2, TAR RNA-binding protein 2, tarbp2, Trans-activation-responsive RNA-binding protein, TRBP, TRBP1, TRBP2, TRBP2_HUMAN.

Background: Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR.

Gene ID: 6895

UniProt: Q15633

Pathways: Regulatory RNA Pathways, Ribonucleoprotein Complex Subunit Organization