ALKBH5 antibody (AA 1-100) (Biotin)

Catalog No. ABIN6982748

Product Details anti-ALKBH5 Antibody (Biotin)

Target: ALKBH5 (AlkB, Alkylation Repair Homolog 5 (ALKBH5))

Binding Specificity: AA 1-100

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALKBH5 antibody is conjugated to Biotin

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ALKBH5

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for ALKBH5

Target: ALKBH5 (AlkB, Alkylation Repair Homolog 5 (ALKBH5))

Alternative Name: ALKBH5 (ALKBH5 Products)

Synonyms: RGD1309496 antibody, ABH5 antibody, OFOXD antibody, OFOXD1 antibody, AW050020 antibody, AW215868 antibody, Abh5 antibody, E130207K11 antibody, Ofoxd antibody, alkB homolog 5, RNA demethylase antibody, alkB homolog 5, RNA demethylase L homeolog antibody, Alkbh5 antibody, alkbh5.L antibody, ALKBH5 antibody

Background:

Synonyms: ABH5, AlkB, alkylation repair homolog 5 (E. coli), AlkB, alkylation repair homolog 5, Alkylated DNA repair protein alkB homolog 5, Alpha ketoglutarate dependent dioxygenase alkB homolog 5, OFOXD, OFOXD1, Oxoglutarate and iron-dependent oxygenase domain containing, Probable alpha ketoglutarate dependent dioxygenase ABH5, RNA demethylase ALKBH5.

Background: Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A)RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24778178,PubMed:24616105, PubMed:24489119). Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro)(PubMed:24616105). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24778178,PubMed:24616105, PubMed:24489119). Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736). Required for spermatogenesis (By similarity).

Gene ID: 54890

UniProt: Q6P6C2