VRK2 antibody (AA 151-250) (AbBy Fluor® 488)
-
- Target See all VRK2 Antibodies
- VRK2 (Vaccinia Related Kinase 2 (VRK2))
-
Binding Specificity
- AA 151-250
-
Reactivity
- Mouse
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This VRK2 antibody is conjugated to AbBy Fluor® 488
-
Application
- Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- Cross-Reactivity
- Mouse
- Predicted Reactivity
- Human,Rat,Chicken
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human VRK2
- Isotype
- IgG
- Top Product
- Discover our top product VRK2 Primary Antibody
-
-
- Application Notes
-
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- VRK2 (Vaccinia Related Kinase 2 (VRK2))
- Alternative Name
- VRK2 (VRK2 Products)
- Synonyms
- 2810003O05Rik antibody, AI447698 antibody, vaccinia related kinase 2 antibody, VRK2 antibody, Vrk2 antibody
- Background
-
Synonyms: Serine/threonine-protein kinase VRK2, VRK2, Vaccinia-related kinase 2
Background: Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant. Isoform 2 phosphorylates 'Thr-18' of p53/TP53, as well as histone H3. Reduces p53/TP53 ubiquitination by MDM2, promotes p53/TP53 acetylation by EP300 and thereby increases p53/TP53 stability and activity.
- Gene ID
- 7444
- UniProt
- Q86Y07
-