SUPT16H antibody

Catalog No. ABIN6972824

Product Details anti-SUPT16H Antibody

Target: SUPT16H (FACT complex subunit SPT16 (SUPT16H))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUPT16H antibody is un-conjugated

Application: Western Blotting (WB)

Characteristics: Supt16H / Supt16 (suppressor of Ty 16 homolog (S. cerevisiae)) is a component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene (By similarity). Supt16H / Spt16 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot, it has been shown to react with Human samples.

Purification: Affinity Purified

Immunogen: This antibody was raised against a synthetic peptide containing human Supt16H / Spt16.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for SUPT16H

Target: SUPT16H (FACT complex subunit SPT16 (SUPT16H))

Alternative Name: Supt16H / Spt16 (SUPT16H Products)

Synonyms: CDC68 antibody, FACTP140 antibody, SPT16/CDC68 antibody, CG1828 antibody, DRE4/dSPT16 antibody, Dmel\\CG1828 antibody, SPT16 antibody, Spt16 antibody, dSPT16 antibody, dSpt16 antibody, dspt16 antibody, l(3)62Bg antibody, l(3)dre4 antibody, spt16 antibody, Cdc68 antibody, Fact140 antibody, Supt16h antibody, T12H20.3 antibody, T12H20_3 antibody, global transcription factor C antibody, SSF1 antibody, DDBDRAFT_0204912 antibody, DDBDRAFT_0231753 antibody, DDB_0204912 antibody, DDB_0231753 antibody, DUF140 antibody, SPT16 homolog, facilitates chromatin remodeling subunit antibody, FACT complex subunit spt16 antibody, FACT complex subunit SPT16 antibody, CG1828 gene product from transcript CG1828-RB antibody, suppressor of Ty 16 antibody, global transcription factor C antibody, chromatin-remodeling protein SPT16 antibody, FACT complex component Spt16 antibody, chromatin-remodeling protein antibody, global regulator of transcription antibody, FACT (FAcilitates Chromatin Transcription) complex subunit, putative antibody, SPT16 homolog, facilitates chromatin remodeling subunit S homeolog antibody, SUPT16H antibody, AOR_1_1808194 antibody, CC1G_05155 antibody, dre4 antibody, Supt16 antibody, SPT16 antibody, spt16 antibody, CDC68 antibody, Supt16h antibody, supt16h antibody, supt16h.S antibody

Molecular Weight: 140 kDa

NCBI Accession: NP_009123

Pathways: Chromatin Binding