SOX2 antibody (C-Term)

Catalog No. ABIN6972778

Product Details anti-SOX2 Antibody

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP)

Characteristics: Sox2 (SRY related HMG BOX gene 2) is a DNA binding transcription factor and a member of the SOX family of proteins. SOX proteins have an HMG box that binds DNA. Sox2 forms a complex with Oct-4 and controls the expression of a number of genes involved in embryonic. Sox2 is critical for early embryogenesis and for embryonic stem cell pluripotency and thus can serve as a stem cell marker. Overexpression of Sox2 (along with Oct-4, KLF4 and c-Myc) can transform mouse fibroblasts into a state resembling embryonic stem cells (ES cells), referred to as Induced Pluripotency. Defects in Sox2 are the cause of microphthalmia syndromic type 3. Sox2 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human and Mouse samples.

Purification: Affinity Purified

Immunogen: This Sox2 antibody was raised against a peptide within the C-terminal region of human Sox2.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

References for anti-SOX2 antibody (ABIN6972778)

Janssens, Wu, Sarthy, Meers, Myers, Olson, Ahmad, Henikoff: "Automated in situ chromatin profiling efficiently resolves cell types and gene regulatory programs." in: Epigenetics & chromatin, Vol. 11, Issue 1, pp. 74, (2019) (PubMed).

Target Details for SOX2

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Alternative Name: Sox2 (SOX2 Products)

Synonyms: ANOP3 antibody, MCOPS3 antibody, RGD1565646 antibody, Sox-2 antibody, lcc antibody, ysb antibody, cb236 antibody, wu:fb83g04 antibody, wu:fc14d07 antibody, zgc:65860 antibody, zgc:77389 antibody, SRY-box2 antibody, Xsox-2 antibody, anop3 antibody, mcops3 antibody, sox-2 antibody, SRY-box 2 antibody, SRY box 2 antibody, SRY (sex determining region Y)-box 2 antibody, SRY-box 2 S homeolog antibody, SOX2 antibody, Sox2 antibody, sox2 antibody, sox2.S antibody

Molecular Weight: 42 kDa

NCBI Accession: NP_003097

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis