SMC1A antibody (C-Term)

Catalog No. ABIN6972754

Product Details anti-SMC1A Antibody

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMC1A antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq)

Characteristics: The Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. SMC1 has a myosin-like ATPase domain that serves as a molecular motor to help organize chromatin and is part of the cohesin complex that facilitates chromosome cohesion during the cell cycle. SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells. SMC1 is also involved in DNA damage repair. Subsequent to double strand DNA breaks, SMC1 is phosphorylated by the ATM kinase. Phosphorylated SMC1 is crucial to the successful repair of DNA damage. Defects in SMC1 isoform A are the cause of Cornelia de Lange syndrome type 2, an inherited developmental disorder associated with malformations affecting multiple systems. SMC1-a antibody (pAb) was raised in a Rabbit host. It has been validated for use in ChIP-Seq, Immunoprecipitation and Western blot, it has been shown to react with Human samples.

Purification: Affinity Purified

Immunogen: This SMC-α antibody was raised against a peptide within the C-terminal region of human SMC1-α.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for SMC1A

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Alternative Name: SMC1-α (SMC1A Products)

Synonyms: CDLS2 antibody, DXS423E antibody, SB1.8 antibody, SMC1 antibody, SMC1L1 antibody, SMC1alpha antibody, SMCB antibody, SMC-1A antibody, NV15570 antibody, 5830426I24Rik antibody, Sb1.8 antibody, Smc1 antibody, Smc1alpha antibody, Smc1l1 antibody, Smcb antibody, mKIAA0178 antibody, smc1 antibody, smc1a antibody, smc1l1 antibody, xSMC1 antibody, smc1aa antibody, structural maintenance of chromosomes 1A antibody, structural maintenance of chromosomes 1A b antibody, structural maintenance of chromosomes 1A L homeolog antibody, SMC1A antibody, smc1a antibody, Smc1a antibody, smc1ab antibody, smc1a.L antibody

Molecular Weight: 160 kDa

NCBI Accession: NP_006297

Pathways: Stem Cell Maintenance