Rpb1 CTD antibody (pSer5)

Catalog No. ABIN6972642

Product Details anti-Rpb1 CTD Antibody

Target: Rpb1 CTD (RNA Polymerase II CTD)

Binding Specificity: pSer5

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Rpb1 CTD antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Characteristics: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at Serine 5 and Serine 2. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription. RNA pol II CTD phospho Ser5 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot, it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.

Purification: Protein A Chromatography

Immunogen: This RNA pol II CTD phospho Ser5 antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at serine 5.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for Rpb1 CTD

Target: Rpb1 CTD (RNA Polymerase II CTD)

Alternative Name: RNA pol II CTD (Rpb1 CTD Products)

Molecular Weight: 210 kDa

NCBI Accession: NP_000928