Rpb1 CTD antibody (pThr4)

Catalog No. ABIN6972631

Product Details anti-Rpb1 CTD Antibody

Target: Rpb1 CTD (RNA Polymerase II CTD)

Binding Specificity: pThr4

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Rpb1 CTD antibody is un-conjugated

Application: Western Blotting (WB)

Characteristics: RNA pol II (RNA polymerase II) is a complex of 12 proteins that participates in transcription, mRNA processing, and transcription-coupled DNA repair. RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain (CTD), composed of repeats of the consensus heptapeptide Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Polo-like kinase 3 (plk3) has been reported to phosphorylate Thr4 which may be required for transcriptional elongation. RNA pol II CTD phospho Thr4 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot, it has been shown to react with Human samples.

Purification: Protein A Chromatography

Immunogen: This RNA Pol II CTD phospho Thr4 antibody was raised against a peptide containing the RNA Pol II CTD sequence phosphorylated at threonine 4.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for Rpb1 CTD

Target: Rpb1 CTD (RNA Polymerase II CTD)

Alternative Name: RNA pol II CTD (Rpb1 CTD Products)

Molecular Weight: 240 kDa

NCBI Accession: NP_000928