PAF1/PD2 antibody

Catalog No. ABIN6972477

Product Details anti-PAF1/PD2 Antibody

Target: PAF1/PD2 (PAF1) (Paf1, RNA Polymerase II Associated Factor, Homolog (PAF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAF1/PD2 antibody is un-conjugated

Application: Western Blotting (WB)

Characteristics: PAF1 (RNA polymerase II-associated factor 1 homolog) is a component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1, it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1), UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors. Has oncogenic activity in vivo and in vitro. PAF1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot, it has been shown to react with Human samples.

Purification: Affinity Purified

Immunogen: This antibody was raised against a peptide within human PAF1.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for PAF1/PD2

Target: PAF1/PD2 (PAF1) (Paf1, RNA Polymerase II Associated Factor, Homolog (PAF1))

Alternative Name: PAF1 (PAF1 Products)

Synonyms: F23149_1 antibody, PD2 antibody, 5730511K23Rik antibody, AA675045 antibody, f23149_1 antibody, pd2 antibody, im:6906070 antibody, paf1l antibody, wu:fb80a09 antibody, paf1 antibody, RGD1306219 antibody, PAF1 homolog, Paf1/RNA polymerase II complex component antibody, Paf1, RNA polymerase II complex component antibody, PAF1 homolog, Paf1/RNA polymerase II complex component L homeolog antibody, RNA polymerase II-associated factor 1 homolog pseudogene antibody, RNA polymerase II-associated factor 1 homolog antibody, paf1, RNA polymerase II associated factor, homolog (S. cerevisiae) antibody, Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae) antibody, PAF1 antibody, Paf1 antibody, paf1 antibody, paf1.L antibody, LOC100019208 antibody, LOC100161171 antibody, LOC100639019 antibody, LOC100650306 antibody

Molecular Weight: 65 kDa

NCBI Accession: NP_061961

Pathways: Cellular Response to Molecule of Bacterial Origin, Stem Cell Maintenance