OCT4 antibody

Catalog No. ABIN6972459

Product Details anti-OCT4 Antibody

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP)

Characteristics: Oct-4 (Octamer binding protein-4, POU5F1) is a member of the POU family of transcriptional activators. Oct-4 is critical for early embryogenesis and required for embryonic stem cell pluripotency. Oct-4 is expressed at high levels in undifferentiated cells and ectopic expression of Oct-4 (and several other transcription factors) can induce pluripotency in differentiated cells. Oct-4 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human and Mouse samples.

Purification: Affinity Purified

Immunogen: This Oct-4 antibody was raised against a peptide derived from human Oct-4.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: Oct-4 (POU5F1 Products)

Molecular Weight: 45 kDa

NCBI Accession: NP_002692

Pathways: Stem Cell Maintenance