Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Characteristics
LIN28 is an evolutionarily conserved RNA-binding protein that serves as a marker of undifferentiated human embryonic stem cells. LIN28 can facilitate the reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs). LIN28A antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human and Mouse samples.
Purification
Affinity Purified
Immunogen
This LIN28A antibody was raised against a peptide derived from the C-terminal region of human LIN28A.
LIN28A
Reactivity: Human
IF, DB
Host: Rabbit
Polyclonal
RB18937
unconjugated
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Buffer
Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.