MLL5/KMT2E antibody

Catalog No. ABIN6972268

Product Details anti-MLL5/KMT2E Antibody

Target: MLL5/KMT2E (MLL5) (Myeloid/lymphoid Or Mixed-Lineage Leukemia 5 (Trithorax Homolog) (MLL5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLL5/KMT2E antibody is un-conjugated

Application: Western Blotting (WB)

Characteristics: KMT2E (MLL5) (myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila / lysine N-methyltransferase 2E)) is a histone methyltransferase that specifically mono- and dimethylates 'Lys-4' of histone H3 (H3K4me1 and H3K4me2). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation. Plays an essential role in retinoic-acid-induced granulopoiesis by acting as a coactivator of RAR-alpha (RARA) in target gene promoters. Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages. Required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells. Overexpression inhibits cell cycle progression, while knockdown induces cell cycle arrest at both the G1 and G2/M phases. KMT2E (MLL5) antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot, it has been shown to react with Human and Mouse samples.

Purification: Affinity Purified

Immunogen: This antibody was raised against a peptide within human KMT2E (MLL5).

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for MLL5/KMT2E

Target: MLL5/KMT2E (MLL5) (Myeloid/lymphoid Or Mixed-Lineage Leukemia 5 (Trithorax Homolog) (MLL5))

Alternative Name: KMT2E (MLL5) (MLL5 Products)

Synonyms: HDCMC04P antibody, MLL5 antibody, 1810033J14Rik antibody, 9530077A04Rik antibody, D230038D11Rik antibody, Mll5 antibody, lysine methyltransferase 2E antibody, lysine (K)-specific methyltransferase 2E antibody, KMT2E antibody, Kmt2e antibody

Molecular Weight: 85 kDa

NCBI Accession: NP_891847

Pathways: Retinoic Acid Receptor Signaling Pathway, Warburg Effect