Histone H3.1 antibody (AA 21-39)

Catalog No. ABIN6971835

Product Details anti-Histone H3.1 Antibody

Target: Histone H3.1 (HIST1H3B)

Binding Specificity: AA 21-39

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H3.1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq)

Characteristics: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. There are three protein variants of Histone H3, Histone H3.1, 3.2 and 3.3. The incorporation of Histone H3.1 and H3.2 into nucleosomes is replication dependent, in contrast to Histone H3.3, which is independent of DNA synthesis and occurs throughout the cell cycle. Human Histone H3.1 and H3.2 are identical in amino acid sequences except at position 110 where H3.1 has a cysteine and H3.2 has a serine. Histone H3.1 / 3.2 antibody (mAb) (Clone 1D4F2) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a peptide comprising amino acids 21-39 of human Histone H3.1. This region is 100% identical in human Histone H3.2.

Clone: 1D4F2

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for Histone H3.1

Target: Histone H3.1 (HIST1H3B)

Alternative Name: Histone H3.1 / 3.2 (HIST1H3B Products)

Synonyms: H3 histone antibody, Hist1h2ai antibody, histone cluster 1, H2ai-like1 antibody, histone H3.1 antibody, Hht21p antibody, Hist1h2ail1 antibody, H3.1 antibody, HHT21 antibody

Molecular Weight: 17 kDa

NCBI Accession: NP_003522