Histone H2A antibody (pThr120)

Catalog No. ABIN6971791

Product Details anti-Histone H2A Antibody

Target: Histone H2A

Binding Specificity: pThr120

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB)

Characteristics: Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Phosphorylation of histones occurs at multiple sites during mitosis. H2A Thr120 phosphorylation is observed on chromatin during both mitosis and meiosis. Thr120 phosphorylation is inversely correlated with ubiquitylation of H2A Lys119 in meiotic mouse spermatocytes. In Drosophila, loss of H2A Thr120 phosphorylation is associated with a failure to disassemble the synaptonemal complex, impaired loading of condensin and female infertility. It is possible that H2A Thr120 phosphorylation is involved in the regulation of chromatin structure. Histone H2AT120ph antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot, Immunofluorescence and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Purification: Protein A Chromatography

Immunogen: This Histone H2A phospho Thr120 antibody was raised against a peptide containing phospho Thr120 of human histone H2A.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for Histone H2A

Target: Histone H2A

Abstract: Histone H2A Products

Synonyms: 5499 antibody, CG5499 antibody, Dmel\\CG5499 antibody, H2A antibody, H2A.F/Z antibody, H2A.X antibody, H2A.Z antibody, H2AV antibody, H2AV_DROM antibody, H2AX antibody, H2AZ antibody, H2Av antibody, H2AvD antibody, H2a.V antibody, H2av antibody, H2avD antibody, HIS antibody, HIS2AV antibody, HIS2AVD antibody, His antibody, His2 antibody, His2AV antibody, His2AvD antibody, HisH2Av antibody, Hist antibody, Hist2av antibody, gamma-H2Av antibody, gamma-HIS2AV antibody, gamma-His2Av antibody, gammaH2Av antibody, h2AvD antibody, his antibody, l(3)05146 antibody, l(3)810 antibody, l(3)97Dd antibody, l(3)L1602 antibody, DDBDRAFT_0205974 antibody, DDBDRAFT_0216271 antibody, DDB_0205974 antibody, DDB_0216271 antibody, RGD1564767 antibody, H2a-221 antibody, H2A-III antibody, HIST1H2AA antibody, Histone H2A variant antibody, putative histone H2A antibody, histone H2A antibody, histone cluster 1 H2A family member F antibody, histone H2A-like antibody, histone cluster 1, H2af antibody, histone2A1 antibody, histone cluster 1, H2A, III antibody, histone cluster 1, H2ac antibody, His2Av antibody, LMJF_17_0280 antibody, H2AX antibody, Hist1h2af antibody, LOC100425378 antibody, his2a1 antibody, HIST1H2A3 antibody, HIST1H2AC antibody, h2a-A antibody

Molecular Weight: 14 kDa

NCBI Accession: NP_003508