Histone H1 antibody (AA 109-256)
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- Target See all Histone H1 Antibodies
- Histone H1
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Binding Specificity
- AA 109-256
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Reactivity
- Drosophila melanogaster
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Histone H1 antibody is un-conjugated
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Application
- Immunofluorescence (IF), Western Blotting (WB), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP)
- Characteristics
- The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation and play a major role in regulating gene expression. Histone H1 antibody (mAb) (Clone 1G6-E3) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunofluorescence, Immunoprecipitation and Western blot, it has been shown to react with Drosophila samples.
- Purification
- Protein A Chromatography
- Immunogen
- This antibody was raised against a recombinant protein containing amino acids 109-256 of Drosophila melanogaster Histone H1. This region of the protein is not conserved in mammals, the antibody is not likely to cross-react to human, mouse or rat Histone H1.
- Clone
- 1G6-E3
- Isotype
- IgG1
- Top Product
- Discover our top product Histone H1 Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Buffer
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- Target
- Histone H1
- Abstract
- Histone H1 Products
- Synonyms
- H10 antibody, H1FV antibody, H1 histone family member 0 antibody, H1F0 antibody
- Molecular Weight
- 35 kDa
- NCBI Accession
- NP_001027286
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