HIRA antibody (AA 421-729)

Catalog No. ABIN6971732

Product Details anti-HIRA Antibody

Target: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Binding Specificity: AA 421-729

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This HIRA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Characteristics: HIRA (DGCR1, TUP1) is a histone chaperone that preferentially places the variant Histone H3.3 in nucleosomes, replacing canonical Histone H3 during the cell cycle. HIRA is required for the periodic repression of histone gene transcription during the cell cycle and when complexed with ASF1a is involved in the formation of the senescence-associated heterochromatin foci. HIRA antibody (mAb) (Clone WC119.2H11) was raised in a Mouse host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human and Mouse samples.

Purification: Protein G Chromatography

Immunogen: This HIRA antibody was raised against a GST-fusion protein containing residues 421-729 of human HIRA.

Clone: WC119-2H11

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for HIRA

Target: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Alternative Name: HIRA (HIRA Products)

Molecular Weight: 120 kDa

NCBI Accession: NP_003316

Pathways: Chromatin Binding